We have a problem for the detection of mycoplasma in some cell cultures that we do. For our Expi293F cells (obtained from ThermoFisher), cultivated in the Expi293™ Expression Medium (Gibco), we never get any results on our gel after amplification through PCR. Not negative, not positive, absolutely nothing appears on the gel ...

We use the Venor GeM Classic mycoplasma detection kit from Minerva Biolabs, which works usually fine on every other cultures that we have.

Does anyone have an idea where the problem might come from ?

Thank you so much !

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