This was from a whole cell lysis protocol using 125uL of Y-PER and 2uLof PIC. I agitated the yeast cell pellet twice and then centrifuged the samples.

I added 10uL of 2X loading buffer+BME with 10uL of sample. I boiled them at 95C for 5 minutes and then loaded and ran the gel at 165V for 30min. I ran another gel with a control protein and that ran normally, but these samples ran in the same way so something is happening with these samples in particular.