Behzad Hussain

7 Questions 4 Answers 0 Followers

Questions related from Behzad Hussain

'' The Reviewer has finalized their report and endorsed the publication of this manuscript.'' What does this statement mean?

I submitted my manuscript in the Journal Frontiers in Microbiology and responded to all the comments/questions of the reviewers. Now, both the reviewers have mentioned this statement on the...

07 March 2024 8,292 1 View

Can I use Recombination Identification Program (RIP) tool of HIV LANL database to check recombination of SARS-CoV-2 genomes?

Hello everyone. I am working on SARS-CoV-2 genome analysis and trying to detect recombination in the said genomes, if any. I am curious if the Recombination Identification Program (RIP) tool of...

14 September 2023 4,094 3 View

Do the ELISA OD450nm values of SARS-CoV-2 normally distributed among different groups? What statistical test should I use?

Hello everyone. I have done ELISA using coating antigens from different 'groups'. I am confused about whether the data of OD450nm values are normally distributed. Some of the researchers have used...

04 May 2023 3,689 0 View

While running the SDS-PAGE gel, why does the diffusion happen across the sample wells?

Hello, everyone. I am running SDS_PAGE gel and while running the gel for about 10 minutes, it seems like the sample wells are getting diffused with the blue colour (of the sample loading buffer)....

23 February 2023 7,965 5 View

Can the diluted PEI solution be used after keeping it at room temperature for about 3-4 hours?

I forgot to put the PEI stock solution (10mg/ml) at 4'C or -20'C temperature and left it at room temperature for about 3-4 hours. After that, I put it at -20'C temperature. Can I still use it for...

18 November 2022 7,438 0 View

Why do the HEK293T cells detach from the surface of the 24-well cell culture plate?

When I culture the HEK293T cells in cell culture plates (60 or 100mm ), they grow well but when I culture the cells in 24-well cell culture plate then after one day of incubation at 37'C, the...

16 December 2021 8,351 4 View

On what basis a single amino acid substitution/mutation is to be selected to study a particular function of a protein?

For example: If I have a wild type protein with 'L' amino acid at 174 position and some researchers have changed it to 'P' amino acid but some researchers have changed it to 'D' amino acid. My...

15 November 2021 2,001 1 View