Often yellowing leaves in tissue culture could be due to the depletion of the contents of the nutritional medium due to the long incubation period in the same container and delaying in subculture processes.
I totally agree with Hany Osman, try to make the subculture earlier and check the light intensity of the growth room.
Hany Osman is right.Don't leave cultures for a long time in the culture flasks.
Thanks for all of your comments
How is usage of casein hydrolysate in remove of yellowing of leaves?
@Atefe,
How big were your 'plantlets' when you noticed that their leaves turned yellow? and how often did you subculture your plants? If your plantlets are small, and you subculture them every 2-3 weeks, that was about normal. Then, probably you need to direct your attention to your medium components. Did you follow a successful medium receipt from a published paper. If you are using MS medium, make sure the MS powder you purchased already have vitamins added; otherwise, you have to add it separately. There are all kinds of MS medium powder mix out there. Need to be very careful to choose them.
casein hydrolysate may some time used to enhance the number of multiple shoots, no idea weather it can prevent yellowing
Thank you for your comments.
my plantlets were about 6cm when I noticed that their leaves turned yellow. I do subculture every 4 weeks and the media are normal and I use Ms.
How often you subculture your plantlets. Frequent subculturing may reduce the yellowing.
If plant shoots or long leaves touches to the tissue culture glass/ test tube sides, vapors or water drops formed in the bottle/ test tube causes the yellowing of leaves. It happened for my plants in rooting media.
The most of my culture have green and new growth with application BA in media.
Thank you Dr.Dewir
