I have HBMEC cells that are not sticking to the plate. I already increased the fetal bovine serum concentration, but nothing worked. I have already collected the cells that did not adhere and transferred them to a new plate with a higher concentration of fetal bovine serum, and even then it does not get successful. What could be happening?
Dear Géssica!
Please You read this article:
Article Establishment of primary cultures of human brain microvascul...
Please You read this article.
This isn't about the FBS.
Try using plates or Petri dishes with increased adhesion (for example, I used Petri dishes).
Also, if cells do not adhere well, plates or Petri dishes covered with collagen or fibronectin can be used.
Hello Gessica
In addition to what Alexandr has mentioned I would also like to add a few points. Cells may fail to attach because of conditions that induce stress. Stress can be experienced by cells in many ways like insufficient nutrients in the culture medium, presence of contamination, inappropriate ways of thawing the frozen vial of cells, at the time of subculturing during trypsinization and so on and so forth.
Regards.
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