I need to measure if my mice have muscle pain after exercise. I'm not sure which test to do, I was thinking of running them on a treadmill first to simulate the exercise, but then I'm not sure what to do exactly. maybe a Von Frey but it's indirect. a PAM? I saw images of pam at the level of the thigh to press on the muscle but in the rat, in the mouse it seems to me impossible to do. How to hold the mouse, apply pressure on the thigh, leaving it enough freedom to express the signs of pain?
thank you in advance for your suggestions :)
Article An Overview of Animal Models of Pain: Disease Models and Out...
Article Experimental Animal Models of Muscle Pain and Analgesia
There are a few reviews on this subject such as these attached that should help you get some ideas. It does look like some of the models you mentioned have been used in the past. Also, some models such as those measuring grip strength following exercise or muscle injuries have been used. I think there is also something to be said for measuring reductions in innate behaviors following the muscle pain induction. For example, do the mice exhibit reduced locomotor behavior in open field test, or reductions in nest building? That might be something comparable to people "not being able to walk" (or otherwise very uncomfortable) the day after a tough workout.
Best,
Justin