Hi all,
I want to express several genes in S.aureus by using the pRMC2 expression vector. I want to try to subclone some genes already cloned into pRMC2 (and their own tetO promoter) and put them together in a new pRMC2 plasmid. With this method, I hope to be able to express several genes from the same plasmid. Do you think that it can be done? My principal question is that, do you think that the tetR regulator will be produced in sufficient level to repress all genes under tetO promoter ?
Thank you for your answers.
Since tetR gene is on the plasmid and therefore will be present in multi copy, there should be ample TetR to regulate the promoters.
Is there a reason why you're including the separate tet promoters rather than just cloning them in as a whole operon with one promoter?
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