I have CRISPR clones which I am doing a variety of cell viability assays. I have already done cell counting and plan on doing one or two more to validate. Would MTS be appropriate? I am not doing a treatment, which means I do not have a "zero hour" time point to normalize the raw data values. My plan was to plate the cells, and collect readings ever 24hrs for 4 days. Should I change my design or perhaps look into a different assay altogether? Thank you.
Hello Harro,
You can follow indirectly the growth of your culture with a MTS or MTT. You don't have to expose your clones to a treatment for that. You can show the optic density (OD) instead of percentage of control, or you can normalize your data with your first time point "zero hour".
About your design, don't forget to add a well without clones, just fill with the culture media. It will be your blank. You also have to be sure to seed enough clones at the beginning to be able to detect enough signal (above your blank), but be sure to don't seed too much clones for not saturated your MTS signal and the end of your experiment (or reduce the time of your experiment). And of course, take pictures!
Good luck!
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