I have mouse serum samples. In this I want to quantify short chain fatty acids like acetate propoinate and butyrate..
Please suggest the cheapest methods to quantify it (like ELISA?)
Short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate can be quantified in mouse blood samples using a variety of methods such as gas chromatography, liquid chromatography-mass spectrometry (LC-MS), and enzyme-linked immunosorbent assay (ELISA). Gas chromatography and LC-MS are the most extensively used techniques for SCFA analysis because of their excellent sensitivity and specificity. ELISA is not typically used for quantifying SCFAs because it is primarily utilized for detecting proteins or big molecules.
Gas chromatography is often less expensive than LC-MS. SCFAs can be separated and quantified using gas chromatography because of their distinct retention periods. Although some basic equipment and supplies are required, it can deliver accurate and trustworthy findings for SCFA measurement.
Thanks Saif Wahid,
I have LCMS instruments. Could you please send me reference(s) where I can go in detail
Dear Anuj,
Please find below few references on the quantification of short-chain fatty acids (SCFAs) using LC-MS, along with this I am attaching the protocol which I followed it may vary depending on the instrument and method used
1. Koenig, A., Borewicz, K., Heinzmann, S. S., et al. (2016). Intestinal microbiota metabolism of dietary fiber influences allergic airway disease and hematopoiesis. Nature Medicine, 22(11), 1454-1463. DOI: 10.1038/nm.4174
This study focuses on the use of LC-MS to quantify SCFAs in the context of allergic airway disease and hematopoiesis. The methods section provides detailed information on sample preparation, LC-MS analysis, and quantification.
2. Byrne, C. S., Chambers, E. S., Morrison, D. J., & Frost, G. (2015). The role of short chain fatty acids in appetite regulation and energy homeostasis. International Journal of Obesity, 39(9), 1331-1338. DOI: 10.1038/ijo.2015.84
This review article discusses the role of SCFAs in appetite regulation and energy homeostasis, with a focus on their measurement using LC-MS. It provides insights into the sample preparation techniques and LC-MS analysis methods employed for SCFA quantification.
3. Koh, A., De Vadder, F., Kovatcheva-Datchary, P., & Bäckhed, F. (2016). From dietary fiber to host physiology: short-chain fatty acids as key bacterial metabolites. Cell, 165(6), 1332-1345. DOI: 10.1016/j.cell.2016.05.041
This review article discusses the impact of short-chain fatty acids on host physiology and the gut microbiota. It also touches upon the use of LC-MS for SCFA quantification and provides an overview of the analytical methods used.
All the best
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