We've been looking a kit to measure the amount of endotoxin in mouse serum (with high-fat diet or not). What's the most reliable and practical kit for this? I found a thread about this but it has been a year. We also looked into the papers and some used QCL-1000 from LONZA. Another new kit is EndoLISA. Does anybody have hands-on experience on any of these? Thanks for your suggestions.
https://www.researchgate.net/post/Can_anyone_recommend_a_test_for_plasma_endotoxin_measurement
We can not make a specific recommendation, but we can suggest a number of suppliers of ELISA kits for Mouse Endotoxin detection (including EndoLISA) for you to explore.
Here are some listings: http://www.linscottsdirectory.com/products?q=mouse%20endotoxin (select Kits: Detection... from Product Type filter).
These cell lines are quite reliable to detect LPS and can be used on mouse plasma/serum
http://www.invivogen.com/hek-blue-tlr4
we have hands-on experience on measure the amount of endotoxin in mouse serum
we used the following kit.it was good
Mouse Lipopolysaccharides(LPS） ELISA Kit cat.NO: CSB-E13066m
supplied by Genxbio Health Sciences Pvt. Ltd.
www.cusabio.com
the aforementioned LAL and ELISA tests are a very effective means at measuring endotoxin. But I would also be wary of your materials used when performing these types of assays because they must remain endotoxin free. You may have to purchase endotoxin free tubes or waters to avoid contamination.
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