I am looking for a way to prepare a mounted bio sample for testing superresolution methods, such as fixed cell culture. So far, most protocols I found for Alexa647 and other dyes require liquid buffers. One exception is Vectashield but it is not perfect either:
Article Effect of Vectashield-induced fluorescence quenching on conv...
What would be the simplest demo sample for physicists who want to learn superresolution bioimaging?
Hi Monika,
What type of super-resolution method you are planning to use? There are a few mentions about different types of dyes:
- https://www.researchgate.net/post/What-can-I-use-as-a-test-sample-for-PALM-STORM-super-resolution
- https://hcbi.fas.harvard.edu/files/hcbidoug/files/en_41_011_065_elyra_sample-prep-quickguide.pdf
- http://www.gattaquant.com/products/localization-based/gatta-paint-nanoruler.html
- 3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples. Methods, Volume 174, 1 March 2020, Pages 56-71.
- Progress in Super-resolution Fluorescence Microscopy. Methods. Edited by Jan TønnesenVolume 174, Pages 1-114 (1 March 2020)
Good luck.
@Sathya Srinivasan
Sathya Srinivasan actually I was thinking of the fluctuation-based methods (SOFI family). There is not so much published there. Thanks for the links!
Check this latest article:
Fluorescence fluctuations-based super-resolution microscopy techniques: an experimental comparative study.
Ida S. Opstad, Sebastian Acuña, Luís Enrique Villegas Hernandez, Jennifer Cauzzo, Nataša Škalko-Basnet, Balpreet S. Ahluwalia, Krishna Agarwal
https://arxiv.org/ftp/arxiv/papers/2008/2008.09195.pdf
Not sure if this article is available for everyone and hope this covers what you are looking for.
There is a RG discussion on it, I think from the authors of the above article:
Preprint Fluorescence fluctuations-based super-resolution microscopy ...
@Sathya Srinivasan
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