With what rationale to choose transwell insert pore size comparing migratory potential of different cell lines?

Hi every one.... We are intending to do some comparative migration/invasion assays in different cell lines- HCT 116, Sw480, HT29 and Colo205. I couldn't find the cell size, so I wanted to know...

10 November 2019 8,990 3 View

The best way to add selection antibiotic to the transfected cells to achieve stable transfection?

Hi every one.... I am looking for some valuable tips to achieve stable transfection in my cells. I have transfected the cells (using lipofectamine 3000) 36 hours ago and as I have read some...

31 December 2018 805 3 View

Reason why my Plasmids isolated using alkaline lysis didn't dissolve in water?

Hi everyone I isolated some plasmids, using alkaline lysis method (which I have been following for 2-3 years now) and the final plasmid pellet after washing in 70% ethanol was dried. Then I added...

11 December 2018 6,516 3 View

I have a query regarding co-transfecting two or more plasmids for stable cell generation?

Actually I intend to do some knockdown and over expression experiments. There are a few queries- 1. I want to generate stably transfected cells in spite of transient ones just to save time and...

04 May 2018 1,647 4 View

What is the best cell confluence at which we should treat our running cancer cells with CoCl2 to mimic hypoxia mediated conditions?

Hello.... I am intending to mimic hypoxia induced conditions in cancer cells with CoCl2 (for 24 hrs) and then re-oxygenate the CoCl2 treated cells with fresh culture media without CoCl2. I am...

03 April 2017 1,650 1 View

Hello, can anyone assist me about the exact conditions to develop a western blot, using Luminata forte?

I just need an assistance about how to develop a blot , using luminata forte and Xray cassete? Like the conditions in which the developer, fixer or the luminata forte to be opened... Is complete...

09 October 2016 3,180 4 View

What can be the reason of not getting any ban blotnd after developing a wester

Hi all I have been facing a lot of problems with my western blot development. 1) First I failed to develop my target protein on x-ray although every time I got a fine band for my control(beta...

01 January 1970 3,179 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View