Rayees Padder

8 Questions 12 Answers 0 Followers

Questions related from Rayees Padder

With what rationale to choose transwell insert pore size comparing migratory potential of different cell lines?

Hi every one.... We are intending to do some comparative migration/invasion assays in different cell lines- HCT 116, Sw480, HT29 and Colo205. I couldn't find the cell size, so I wanted to know...

11 November 2019 9,051 3 View

The best way to add selection antibiotic to the transfected cells to achieve stable transfection?

Hi every one.... I am looking for some valuable tips to achieve stable transfection in my cells. I have transfected the cells (using lipofectamine 3000) 36 hours ago and as I have read some...

01 January 2019 880 3 View

Reason why my Plasmids isolated using alkaline lysis didn't dissolve in water?

Hi everyone I isolated some plasmids, using alkaline lysis method (which I have been following for 2-3 years now) and the final plasmid pellet after washing in 70% ethanol was dried. Then I added...

12 December 2018 6,566 3 View

I have a query regarding co-transfecting two or more plasmids for stable cell generation?

Actually I intend to do some knockdown and over expression experiments. There are a few queries- 1. I want to generate stably transfected cells in spite of transient ones just to save time and...

05 May 2018 1,697 4 View

What is the best cell confluence at which we should treat our running cancer cells with CoCl2 to mimic hypoxia mediated conditions?

Hello.... I am intending to mimic hypoxia induced conditions in cancer cells with CoCl2 (for 24 hrs) and then re-oxygenate the CoCl2 treated cells with fresh culture media without CoCl2. I am...

04 April 2017 1,700 1 View

Most reliable way to induce hypoxic condition in cancer cells?

Hi I have to execuite some protein expression analysis under hypoxic conditions in cancer cells. Can anybody suggest me the most reliable way to induce and mimic hypoxic conditions in cell...

11 November 2016 8,969 4 View

Hello, can anyone assist me about the exact conditions to develop a western blot, using Luminata forte?

I just need an assistance about how to develop a blot , using luminata forte and Xray cassete? Like the conditions in which the developer, fixer or the luminata forte to be opened... Is complete...

10 October 2016 3,232 4 View

What can be the reason of not getting any ban blotnd after developing a wester

Hi all I have been facing a lot of problems with my western blot development. 1) First I failed to develop my target protein on x-ray although every time I got a fine band for my control(beta...

01 January 1970 3,222 1 View