Monitoring Thermal Denaturation

Prasun Kumar @Prasun_Kumar6

07 July 2017 0 8K Report

Hi All:

Sorry for such a basic question that is bothering me for a while...

The question is: Why do we study thermal denaturation of alpha helical peptides at 222 nm.

I can not provide anything more than this. Sorry for it.

Thank you in advance..

Badges
Science topic

Similar topics
Chemistry
Biochemistry

More Prasun Kumar's questions See All

G-Helix and number of residues in a helix ?

Hi All I have been trying to use "G_helix". However, I am encountering a rather strange situation: I am not able to run the command when the number of residues is less than 20 (say 19).It is...

02 March 2017 1,945 1 View

How do I calculate the angle between two vectors in 3D space using atan2?

I have two vectors in 3D space and I want the angle to be in -pi,pi. Hence intend to use atan2 in place of acos function. It will be a great help, if some body can guide me.

01 February 2017 3,998 3 View

Can someone please help with Clustering 2D dataset?

I have a matrix consisting of 10000 rows and 2 columns. I wanted to cluster my dataset into k number of clusters. I am aware of the K-mean available in MATLAB, but its clustering is based on first...

04 May 2014 746 4 View

Can anyone help with small molecules and their superposition?

I have two small molecules and want to superpose them on each other. Can somebody suggest me some command line program (Linux)?

03 April 2014 2,500 10 View

How can I find the rise per residue in a protein?

Can any body help me with finding the rise per residue in a given protein. I know about one such software HELANAL-Plus. Any other software name and relevant information will be a great help.

03 April 2014 2,069 2 View

Can someone help me with my simulation of Fe2+ ions and protein?

Hi all, I have to run a simulation using GROMACS, which has Fe2+ ions. In GROMACS, the C12 and C6 values for Fe are not defined. Can anybody help me in this regard? Any article or relevant data...

02 March 2014 6,541 10 View

Is there any software available to identify the central strands and end strands of a beta sheet?

Is there any software available to identify the central strands and end strands of a beta sheet or to identify the parallel and anti-parallel beta sheets?

07 August 2013 8,212 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View