Dear Experts,
I found in Protein Data Bank PDF file of the protein of interest. But I need to test its analog that differs in of one amino acid. Unfortunately, that analog is not in PDB bank. Do you know how to edit MOL/PDF file to change the sequence of amino acids ?
Thanks?
Ondrej
Hi Ondrej,
You can upload the PDB file into Coot or PyMol and mutate the residue. This will change the amino acid in the uploaded PDB file to your desired amino acid. Save the mutated model into a new .pdb file.
Thanks Nicholas!
I'll try. Do I need some specific plug-in for the mutation in PyMol ?
Thanks.
ondrej
No plug-in required, go to Wizard -> Mutagenesis. Select the amino acid you want to mutate, and in the toolbox on the right, you can change the amino acid. Click apply to mutate the residue.
Thanks, I found it. Can I also Add some amino acid to carbon instead of the hydrogen?
O.
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