In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

What's the best kit to test plasma corticosterone and ACTH concentrations in mice ?

Hi, I'm planning to collect plasma from the tail of anesthetized mice. I am looking for the best method to determine corticosterone and ACTH concentrations in these samples ? Thank you for...

07 September 2020 8,885 4 View

Does anyone know where you can purchase porcine ACTH in powder form?

I am looking to purchase porcine ACTH (for use in birds). Sigma Xi appears to be the main supplier but they are...

31 December 2018 8,083 1 View

What is the problem in this SDS-PAGE result for secreted protein ?

Hi everyone, I am doing western blot for POMC/ACTH secreted from AtT20 cells. The problem is as seen in the picture I added. First 3 lanes are for lysates and not a problem. But the next lanes...

09 April 2018 1,356 6 View

What is the problem in this SDS-PAGE result for secreted protein ?

Hi everyone, I am doing western blot for POMC/ACTH secreted from AtT20 cells. The problem is as seen in the picture I added. First 3 lanes are for lysates and not a problem. But the next lanes...

03 April 2018 9,047 7 View

Does anyone know of an ACTH receptor (MC2R) positive cell line?

Does anyone know of an ACTH receptor (MC2R) positive cell line? So far I have not been able to find a readily available cell line expression this receptor and that is responsive to ACTH.

20 July 2016 3,206 3 View

Does anyone know of an ACTH receptor (MC2R) positive cell line?

Does anyone know of an ACTH receptor (MC2R) positive cell line? So far I have not been able to find a readily available cell line expression this receptor and that is responsive to ACTH.

06 July 2016 325 4 View

Can someone recommend a plasma ACTH immunoassay to replace Disorin's IRMA assay?

02 March 2015 4,259 4 View

Can anyone recommend a good kit for measuring rat plasma ACTH with a radioimmunoassay (preferably I-125)?

01 February 2015 2,186 5 View

Is ACTH peptide extraction from plasma required for ELISA?

I have performed a footshock stress protocol on rats and I am measuring ACTH from plasma. I have already extracted the plasma and it is frozen. I am preparing to run an ELISA on the samples using...

09 September 2014 2,384 2 View

Is ACTH peptide extraction from plasma required for ELISA?

I have performed a footshock stress protocol on rats and I am measuring ACTH from plasma. I have already extracted the plasma and it is frozen. I am preparing to run an ELISA on the samples using...

08 September 2014 7,093 2 View