06 October 2017 1 8K Report

It is known that treatment with cortisol or ACTH causing the eosinopenia and neutrophilia. But through what mechanism it reduces the eosinophils? What is the non-genomic effect of corticosteroids? 

More Ravjibhai Karshanbhai Chaudhari's questions See All
Which has major role in nutrition, Prebiotics or probiotics?

It Seems that the major role is on pre-biotics and its supplement. But as far as the gut health is concerned there is major role of Pro-biotics also. So, i would like to know the expert opinions...

30 January 2024 6,682 4 View

Box-Behnken design in Response Surface Design got result ?

why Lack of Fit P-values show star *

01 January 2024 330 3 View

What is the importance of Dilation Angle in ABAQUS simulation software?

I am working on ABAQUS to simulate the behavior of a reinforced concrete deep beam. I am facing the issue of the Dilation Angle in that. Can anyone please guide me to solve this issue?

03 May 2023 8,009 0 View

How to get y+ value in Ansys CFX ?

I am working on simulation of savonius hydrokinetic turbine in cfx. I have set inflation layer on turbine as shown in photos. It is observed in the previous investigation that, the non-dimensional...

23 April 2023 2,983 3 View

Does letter to editor has same weightage as of Publication?

As there is section, Letter to Editor, does it carries equal weightage as the regular research paper.

03 April 2023 6,580 1 View

How to do methanolic extract of plant oil?

I am taking half quantity of oil and half quantity of methanol, than vertex for 10 min to mix it. Than the upper methanolic portion collecting in other tube or vessel and keeping for methanol...

19 March 2023 5,413 0 View

What are the C4 plants and how they are useful in Tropical Countries?

I want to know about the C4 pathway of Plants and the usefulness of C4 plants in the present scenario where consistent Geo-climatic changes are occurring and role of C4 plants in assuring food...

05 March 2023 399 0 View

Does increasing primer concentration in qPCR increase or decrease the PCR efficiency?

I am trying to develop a standard curve for miRNA using qPCR. I keep getting Ct vs log(mass) slopes around -5, which is much lower than -3.3 (100% PCR efficiency). I am looking for advice on how...

22 February 2023 4,041 3 View

What is the exact difference between Shelf Life and Expiry Date?

Does the Shelf Life is different from Expiry for Drugs as well as Food? If it is different then what is the exact difference?

01 February 2023 6,901 1 View

CAN YOU PLEASE SHARE ON THIS QUERY : What is the physiological basis of plant growth regulator induced hermaphrodite flowers in gynoecious plant?

Gynodioecious cucumber genotype shows induced hermaphrodite flowers post the plant growth regulators spray. What makes a gynoecious plant produce hermaphrodite flowers (fertile maleness in...

09 October 2022 1,032 0 View

Similar questions and discussions
How to set up Mouse neutrophil - T cell coculture in vitro?

Hi, scholars. I am planning to conduct mouse neutrophil and T cell co-culture in Vitro and observe the effect of neutrophils on T cells. I don't have any protocol. Could anyone provide me...

19 June 2024 3,271 2 View

Co-incubation of neutrophils with exosomes?

Hello! I am planning on doing co-incubation experiments of neutrophils with exosomes and wanted to ask what concentration of exosomes, cell number and incubation time works with this cell...

29 May 2024 6,551 0 View

If you have a missing trapezoid in total AUC for salivary cortisol data, is this representative of the cortisol changes to estimate sleep changes?

Participants completed 6 saliva samples throughout the day to capture changes in cortisol and melatonin from waking until just before sleep. To achieve total AUC, 5 trapezoids between samples 1-6...

27 May 2024 6,842 0 View

Is it possible to freeze down freshly extracted bone marrow cells?

So, I want to extract bone marrow from mice for an experiment, but I don't need all tens of millions of cells that I would get. So I was thinking that I could freeze down the left over cells for...

07 May 2024 7,359 1 View

Does BFA keep ROS inside of cells?

I recently discovered that BFA is used in flow cytometry, enabling the observation of intracellular cytokines that would be otherwise released. Is the same principle applicable to ROS? I would...

04 March 2024 7,094 1 View

US-based labs for hair cortisol analysis?

We are looking for labs based in the US that assess cortisol in human hair samples. We used to send samples to Mark Laudenslager's lab at the University of Colorado for analysis. We saw another...

29 February 2024 3,225 0 View

How to determine whether Percoll solution has gone off?

Hi, I am using 2 gradients Percoll protocol to isolate neutrophils from whole blood. I prepare gradients of desired density myself on the day of the experiment from Percoll (Sigma 1644). In my...

28 February 2024 9,499 1 View

How to count neutrophil after isolation from whole blood from human by density gradient centrifugation using GSM1119 and LSM1077?

How to count neutrophil

26 February 2024 5,068 3 View

Good marker for rat blood neutrophils besided RP1?

Hello! I am experiencing some problems in detecting neutrophils in rat blood of F344 rats. I was using RP1 as a marker but somehow the detection fails. I was wondering if there was a good...

04 February 2024 1,918 0 View

How to improve the purity of neutrophils isolated from bone marrow using Histopaque method?

Dear all, Hello, I am recently working on isolating neutrophils from murine bone marrow by using Histopaque 1099 and histopaque 1077, but the purity of the cells are always unsatisfactory. Here I...

01 February 2024 9,203 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close