Can I compare two mean scores of the same instrument, in the same sample? (Paired t-test)?

Hello everyone, I have scores on different scales of a sample of children, and I want to compare the mean scores on the different scales of this sample. For instance, my hypothesis is that...

10 December 2020 2,278 5 View

Protocol for proliferation of CD4+ murine T-cells?

23 July 2019 3,586 7 View

How to dissolve Dasatinib in order to go in vivo?

Dasatinib is an inhibitor of tyrosine kinases. I would like to test the effect of Dasatinib in vivo on my T cells. However, Dasatinib has a very low solubility (0.5mg/ml) with PBS, which is...

21 July 2019 3,056 3 View

Lysing of cell to detect antibody internalization?

07 April 2019 3,293 10 View

Fix 7-AAD staining?

Hi, I would like to make a viability staining using 7-AAD the day before the measurement; Is it possible to fix the cells? If yes, what do you suggest? Thank you!

01 April 2019 4,376 2 View

OT-I Rag1-/- genotyping protocol?

Dear all, Does anyone have a protocol to provide me to genotype OT-I Rag1-/- mouse? I have taken a look to OT-I and Rag1-/- genotyping protocols and I would need 4 primers for Tcra, 4 for Tcrb and...

10 December 2018 2,449 2 View

I need to measure the respiration rates in insects larvae. Do you have any suggestions about a feasible and not expensive equipment that can be used?

I know that there are avaialble some respiration chambers for insects but I was wondering if chambers used for small fruites can be adequate for the scope.

15 October 2017 3,205 2 View

What statistical analysis should I use for hierarchical questions data?

16 November 2015 9,532 4 View

Is it possible to change the orientation of hydrogen atoms of the ligand and protein residues? If yes, how is it possible by using Gromacs 5.0.4?

Dear users, I would like to change the orientation of an hydrogen of a D-serine (my ligand) and of a serine (as protein residue). How is it possible by using Gromacs 5.0.4? Thank you

12 September 2015 6,906 5 View

Could you please recommend me a manufacturer/ vendor of the GLAD(Glancing Angle Deposition) system?

Ideally we would like to buy a PVD equipment that can be used for the layer by layer and columnar growth.

09 June 2015 9,952 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View