1) What are the potential checkpoints that are activated when an obvious mitotic exit delay occurs and why mitotic exit delay differs from cell line to another and from cells treat it with a particular drug to another. For example, if you treat your cells with (x) compound and cells stay in mitosis for 400 minutes and then exit mitosis whilst the control cells stay in mitosis for 60 minutes? Another cell treated with the same drug take about 180 minutes before it exit mitosis whilst its control cells stay only 60 minutes? What are the possible cell fates in each scenario?
2) In time-lapse microscopy we see cells that go at least 2 cell cycles before they die, why? Why the cell would need two cell cycles to die? Are there particular pathways that get activated after a certain period of time? Are there reasons for delayed apoptosis? (note: I'm not generalising here because we knew that some cells only go one cell cycle then die and some go more than two and polyploid then die but I'm specifically talking about those take two cell cycle to die)
Thanks a lot Manuel. Of course it does help a lot, at least you showed insights into whats happening. No, I don't know in which phase of mitosis they are arrested but they take so long time to move on and they take at least 2 cycles before they die, just few of them die from the first one.
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology