Why would someone choose to perform siRNA HTP over small molecule inhibitors HTP or vice versa? If you perform an siRNA screen and end up finding a target and then develop a small molecule inhibitor for that target, then why not from the beginning screens for a small molecule inhibitors? Are there any major advantages or disadvantages of performing one over another?
Because your chemical library might not contain an inhibitor for the target you identify via siRNA.
Also, knowing which target you want to inhibit can allow you to design a more specific chemical screen.
Chemical screens are limited to what chemicals make up you libraries.
siRNA screens are limited in that a particular siRNA may not achieve the required level of knockdown, and maybe you actually want to find a chemical activator rather than an inhibitor, the siRNA can only mimic an inhibitor.
Realistically most of the large chemical libraries that are available have probably been pretty well picked over...
First to be sure to get the 100% inhibition both siRNA and HTA is used. Secondly si RNA inhibit by binding directly to gene thus stoping transcription of gene of choice subsequently translation, while most the HTA or other chemicals bind mostly to protein that subsequently inhibit either transcription or translation. I think this could be possible answer of your query.
Thanks.
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