Mitoracer red CMXRos staining in mictrotiter plate reader protocol?

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I would like to replace the missing values of one year with the average of the other two years: I can't write the corresponding loop ?

I would like to replace the missing values of one year with the average of the other two years: I can't write the corresponding loop

07 December 2020 982 3 View

Why Lactobacillus acidophillus is sensitive to Vancomycin but L. rhamnosus and casei are intrinsically resistant?

Does it have, anything to do with their fermentation status? I know that in several species of LAB, the terminal d-alanine residue is replaced by d-lactate or d-serine in the muramylpentapeptide,...

19 September 2020 3,242 2 View

HeLa cells infected with HPV16/18 or not?

I ant to know if HeLa cells line commonly available in cell culture labs is infected with HPV16/18 or not?

01 September 2020 7,286 3 View

Does anyone have any good documentation for Morris method for Senstitivy analysis ? And does it take intercation between parametrs in consideration ?

Hello everyone, I'm trying to work on a big database for optimizing beam,column and slab sections by taking into consideration their CO2 impact. Many parameters are taken into consideration, and...

09 August 2020 5,296 3 View

How I can cluster statistically bird species that prefer using different layers of the forest trees ?

In the forest birds are using different layers of forest trees from the soil to the canopy. Some birds such as iberian chiffchaffs are using the soil ground to set their nests while other species...

18 July 2020 2,639 1 View

How biological material (Animal carcasses, excrements) collected in field can be best stored for a later DNA and RNA extraction?

How biological materials (Animal carcasses, excrements) collected in field can be best stored for a later DNA and RNA extraction? what is the protocol to follwo and what are the storing...

25 April 2020 5,726 11 View

What u think changing formal education system to innovative ecosystem could bring a positive change to the economy?

which methodology would be best in the thesis carrying topic ":impact of entrepreneurial Ecosystem and innovative education on the students in bringing a societal change:???

18 April 2020 7,042 5 View

How to predict actions from videos using time series analysis?

I was reading your paper on "Human Interaction Prediction Using Deep Temporal Features 2016" ( Human Interaction Prediction Using Deep Temporal Features ) and was trying to implement it on Keras...

30 March 2020 8,925 3 View

Would you kindly send me a name of a good reference for Neural Networks(Artificial Intelligence)?

I want to use it with Matlab

19 March 2020 7,467 5 View

What is the expected time in which COVID-19 (Novel Corona Virus) would vanish from the surface of the Earth?

Dear Fellows With the developing stories of the spread of COVID-19 all around the world, which has been declared as a pandemic by WHO recently, I am wondering, in how much time this virus would...

11 March 2020 3,381 41 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View