Rehan Imad

6 Questions 1 Answers 0 Followers

Questions related from Rehan Imad

HeLa cells infected with HPV16/18 or not?

I ant to know if HeLa cells line commonly available in cell culture labs is infected with HPV16/18 or not?

02 September 2020 7,424 3 View

How many cell lines can be grown in Leibovitz's L-15 medium ?

Does anyone know that how many cells lines and primary culture can be grown in L-15 medium? I need the complete list of cell lines and tissue types. Our CO2 incubator in cell culture facility is...

02 December 2019 4,560 4 View

Mitoracer red CMXRos staining in mictrotiter plate reader protocol?

I need protocol/ publication regarding staining of adherent cells in 96-well plate with Cmxros after treatment with cytotoxic agent and fluorescence measurement by plate reader.

22 January 2019 1,029 2 View

How to avoid cell pellet lose after ethanol fixation?

For TUNEL assay and cell cycle profile analysis, more than half of my pellet gets discarded after washing and removing supernatent after 70% ethanol addition. The cell lines are MCF7 and MDAMB231....

05 March 2018 8,020 3 View

DMSO toxicity against MCF-7 and MDA-MB-231 cell lines as vehicle control?

what is the acceptable limit of DMSO percentage in MCF-7 and MDA-MB-231 for dissolving compounds? i am using 0.2 % DMSO for Annexin V/ PI assay, TUNEL assay and immunocytochemistry and getting a...

02 March 2018 9,590 9 View

RT-PCR for p38 MAPK pathway genes in breast cancer cell lines?

I have some active compounds and I want to check the effect of those compounds on p38 MAPK pathway genes (p38, JNK and ERK1/2) or may by AKT/PKB and PTEN in MCF7 and MDA-MB-231 breast cancer cell...

07 February 2018 6,217 3 View