Hello everyone, and best wishes for 2024!
I need advice on how to identify the vesicles I see after B lymphocyte targeted therapy.
We think it is mitochondria because we can distinguish mitochondrial crests, but it could also be autophagy vesicles (autophagosomes) or lysosomes?
It seems that mitochondria are affected by treatments, but apoptotic and/or necrotic cells suffer mitochondrial damage.
I attached an image (left normal cell and right after treatment)
Do you think it could be oxeioptose? or necroptosis? cells with these vesicles or strongly attached (homotypique adhesion).
Many thanks for your help!
Hello,
Your provided figure has too low quality to see details like double membranes for mitochondria or autophagosomes.
But yes it may be swollen mitochondria... if you don't see anymore normal mitochondria. You pointed also swollen nuclear membrane with white arrow.
Classical autophagosomes with double membrane engulf cytoplasmic material, which is not your case, and it is not lysosomes, which are also full of material and have a small size.
Magnifications are different for the 3 images, which is disturbing at the first look to compare size whit normal mitochondria.
Concerning the different cell deaths, I cannot help much. It doesn't look like apoptosis (condensation and fragmentation of cells) but lower magnifications are needed. Are your cells dying after 4 hours treatment (goal of your treatment)?
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