I am trying to culture staphylococcus aureus in minimal media. So far I have tried DMM, M9 and M63 minimal media which has a carbon source as either glucose or glycerol. Nitrogen source as ammonium sulfate or ferric ammonium citrate or ammonium chloride. DMM contained L-asparagine and M9 and M63 do not have any amino acids.
None of these media are suitable for growth.
Does anyone know of any other media or suggest any improvement on these media?
Thank you
This has been investigated before you will be able to find the recipe here:
Article Factors Affecting Competence for Transformation in Staphyloc...
Hanna Alalam yes suitable paper for answering Khushboo Patel question
Dear Khushboo Patel
citing from the attached paper: "Regardless of the media, it should be noted that S. aureus is auxotrophic for Arginine (Emmett and Kloos, 1979) and Proline (Li et al., 2010), that S. aureus cannot use inorganic sulfur sources (e.g. sulfate or sulfite) (Soutourina et al., 2009), and that S. aureus exhibits a preference for glycolytic carbon sources during aerobic growth (Seidl et al., 2009)"
Hope this will be helpful.
Kind regards
Jan
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