Has anyone disrupted microtubule assembly with nocodazole in mouse bone marrow macrophages? Can you tell me the optimum concentration required and protocol related to it?I am planning to do IF.
I work with a few human cell lines, but not specifically with bone marrow macrophages. However, I'd not expect extreme differences between human cell lines as long as the drug is uptaken. For instance, in HeLa ou U2OS cells, we get full MT depolymerization with 1uM nocodazole treatment and it's quite fast. With 100nM you can already observe some effects and I'd say that the concentrations in between would give you intermediate phenotypes! I hope this is useful.