Luisa Ferreira

5 Questions 5 Answers 0 Followers

Questions related from Luisa Ferreira

Effect of prolonged 2D culturing on CAFs differentiation/markers?

Hi there, I was wondering whether cancer-associated fibroblasts (CAFs), not being cancer cells or immortalised, remain differentiated over several passages in 2D cultures. In the absence of the...

27 January 2023 3,969 2 View

Any experience using simple magnetic beads (protein A or G) for positive cell selection?

Hi, has anyone used simple magnetic beads (used in co-IP assays) to isolate cells from blood samples? Most protocols use streptavidin-magnetic beads for positive selection of labelled cells with...

17 November 2021 1,917 2 View

Culturing monocytes and monocyte-derived macrophages isolated from blood samples - How long do these cells last in culture?

Hi everyone, does anyone know the best condition to isolate and culture monocytes and monocyte-derived macrophages? Many papers suggest DMEM, others RPMI + 10% FBS. I am culturing these cells in...

12 November 2021 8,847 4 View

What can go wrong in a PCR protocol in 24h?

Hi dear colleagues, I am amplifying several fragments between 3500 and 7500 bps from cDNA using fusion polymerase, following the provided protocol for mix preparation and PCR program. I managed...

29 October 2021 3,452 3 View

Curved/deformed spindle microtubules after methanol fixation?

Have anyone experienced curved/deformed spindle microtubules after cold methanol fixation (4-5 minutes, -20C)? Anyone knows what is causing this effect on microtubules? I'm imaging spindle...

12 January 2017 613 1 View