Quantification
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
I would like to calculate the formation energy of P2-Na0.67Fe0.5Mn0.5O2 based on DFT, what should I do step by step. Any help would be appreciated. Thanks.
29 July 2024 8,248 2 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...
18 July 2024 5,602 0 View
Hi there, I have some 16S and metagenomic sequencing data from mice fecals and I want to upload them to NCBI Sequence Read Archive (SRA). I don't know what NCBI package is suitable for my animal...
03 June 2024 9,451 3 View
One of my friends could not register an account on Researchgate with his own email (like gmail). Should we register an account with the school email?
31 May 2024 6,347 3 View
What are the potential complications associated with uncontrolled hyperglycemia in the perioperative setting?The Impact of Hyperglycemia on Perioperative Outcomes in Pat...
07 May 2024 4,190 1 View
My FLUENT model includes fluid and solid regions, with Discrete-Ordinates (DO) radiation model. The heating boundary conditions are defined by a named expression of Temperature-Time for the...
16 April 2024 6,636 3 View
When detecting magnetic Backhausen noise in a DC bias field, why do there be two peaks, one large and one small, in a sinusoidal excitation cycle?
15 April 2024 6,903 0 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...
30 July 2024 8,771 3 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...
28 July 2024 5,410 1 View
Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...
23 July 2024 9,421 3 View
Phase quantification in steel contain BCC and FCC
20 July 2024 8,150 7 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is...
11 July 2024 2,257 1 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower...
11 July 2024 3,557 3 View
I am trying to replicate results published in the journal paper published recently (Modeling of Laser-assisted micro-milling Inconel718 ). In that paper, they used vdflux subroutine to obtain...
05 July 2024 3,701 0 View
