Quantification
Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...
01 February 2021 6,756 4 View
Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...
02 December 2020 9,413 3 View
Respected researchers, I conducted a SEM including 5 variables (two independent variables , two mediators, and a dependent variables), the correlations between these variables range vary from...
01 December 2020 1,338 5 View
I'm trying to test expression of a gene in sorted cells. I plan to extract RNA from sorted cells and to do RT-PCR (reverse transcription PCR). The problem is that from sorting, I can only collect...
21 November 2020 5,467 2 View
As the title has asked. For instence, using metal stick or long glass tube to get rid of the bubbles in columns.
05 November 2020 5,946 4 View
Hi, guys, Do you have problem using B cell negative selection kit form Meltinyi? Sometime the B cell is pure, CD19+>95%, but sometimes it lower than 90%.
19 October 2020 5,160 3 View
I want to do tissue subcellular fractionation of mouse liver. According to the method, the speed to separate ER is over 200,000g for 120 min (Song et al., 2006). However, liver exosome isolation...
12 October 2020 4,929 3 View
how to set up asphalt rheological model (CA and CAM) ? How to build EXCEL?
23 September 2020 7,692 2 View
Hi anyone experienced in studying antibody-antigen binding with biacore (SPR) know should 1:1 binding model be used in kinetics and affinity analysis? Or should we use 1:2(antibody:antigen)? Thanks!
26 August 2020 4,130 3 View
The anthropogenic pratices are getting common these days and many catchments experience water managements e.g., drainage, which has huge impacts on both discharge and water quality dynamics. So...
13 August 2020 1,801 11 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...
02 March 2021 5,829 1 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...
23 February 2021 5,167 3 View
I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...
20 February 2021 6,058 2 View
Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...
15 February 2021 9,922 5 View
I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...
11 February 2021 6,852 4 View
I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...
10 February 2021 6,145 3 View
Hi all, I am looking for some general feedback and information from those that have experience using handheld/portable systems? I am looking at the prospects of using an off the shelf system from...
07 February 2021 9,691 3 View
I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...
04 February 2021 6,580 2 View
