Methylation specific MLPA requirements for HhaI recognition site?

06 July 2022 0 3K Report

I'm looking to detect promoter hypermethylation at a given gene. MS-MLPA looks like a good option but noticed the HhaI recognition site requires the sequence GCGC in order to hybridize and fragment the DNA. My promoter of interest contains one 'GCGC' and I wonder if this is enough for HhaI to exert its effects or multiple GCGC sites are required/beneficial?

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