I want to make knockout strain of Mycobacterium tuberculosis, please suggest available methods.
In the simulation tutorial of the aeroacoustics around a cylinder by ANSYS, recievers are placed at a location where mesh isn't present, how does the solver predict the acoustics at that location...
16 October 2020 3,368 5 View
Hi, I have a problem with cryosections while staining. Positively charged slides are used for cryosections, in spite of that during the staining process, the sections are washed away during the...
10 September 2020 3,767 3 View
I was trying to find out whether my bacteria can degrade crude oil or not using DCPIP method that changes color after oxidizing hydrocarbons. But, I happen to order the crude oil containing 5%...
25 June 2020 9,801 2 View
after miniprep, I sequenced the plasmid. After sequencing I found that a region of plasmid was missing and its size if of around 8kb while it should be of around 12kb. It has happened to me for...
21 March 2020 736 3 View
Hi all, I'm facing problems in staining the cryosection of the cells encapsulated in hydrogels. I'm using both charged slides as well as the albumin coated slides for the HnE staining. During the...
28 May 2019 1,197 5 View
Has anyone done plaque assays for VZV using toluidine stain?
10 March 2019 7,657 1 View
I'm trying to knockdown my protein in adherent cells and label cells with the fucci system for cell-cycle segmentation using microscopy. My protocol is as follows Day 0 : siRNA...
07 August 2018 4,362 4 View
I am currently pursuing Ph.d
01 January 2018 7,222 17 View
Hi all, I wanted to perform ELISA (sandwich elisa) on the protein pellet extracted from cell lysate. I would like to know in which buffer, the protein pellet should be resuspended? Thank you Nisha
10 December 2017 9,325 3 View
Can I get it in matlab format or notepad
08 October 2017 7,788 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.
24 February 2021 4,150 3 View
I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...
21 February 2021 3,838 1 View
I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...
21 February 2021 7,791 3 View
Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...
18 February 2021 2,769 4 View
Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...
17 February 2021 1,012 3 View
Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...
17 February 2021 3,467 4 View