Method/protocol needed

Navjyoti Chakraborty @Navjyoti_Chakraborty

03 March 2013 0 4K Report

Does anyone have a protocol/suggestion on leaching out all forms of N from soil or a method to determine content of nitrate, nitrite or any other form of N present in soil?

Badges
Science topic

More Navjyoti Chakraborty's questions See All

What kind of statistical analysis should be performed?

Suppose I have a wild type and mutants A, B and AB. I take control (untreated) as well as treat each of them (i.e the WT and the mutants A,B and AB) with some treatment/s and perform RT-qPCR for...

10 November 2014 5,117 9 View

What kind of statistical analysis should be carried out for RT-qPCR data, which has been used to validate microarray data?

Suppose I have around 20 differentially expressed genes for which RT-qPCR data has been generated, and the samples are a wild-type plant and a knock-out mutant. Which statistical analysis should...

09 October 2014 2,954 17 View

What is the maximum log2fold change detectable for repressed genes using qPCR?

We need to validate our microarray data for a few down-regulated genes which show log2FC values near -7. Can qPCR detect this level of down-regulation?

03 April 2014 3,543 11 View

Can anyone suggest a tool which can take AGI/TAIR ids as input and map them onto actual pathway maps?

See above

03 April 2014 5,708 2 View

Can someone suggest the controls for GST pull down?

I am attempting GST-pull down assay for finding unknown interacting partners using GST-fusion protein. Normally bait control, prey control, resin control are taken but I am planning to take also...

31 December 2013 10,006 3 View

Has anyone used the Xcelris plant RNA isolation minikit?

I have previously used the qiagen plant RNeasy minikit and that works very well, but I would like to know about the performance of this one.

11 December 2013 1,602 2 View

Nitrate estimation in plants?

Can anyone suggest a reliable method to estimate nitrate content in plant tissue other than kjeldahl method?

04 May 2013 6,048 8 View

Expression of membrane protein

I am trying to express a plant membrane protein using pGEX vector in BL21 strain. I could express the protein but all went into the pellet fraction. Should I purify the protein from the pellet...

01 February 2013 3,164 14 View

Where can I find a protocol for Co-IP for protein-protein interaction?

I am planning to do co-IP for 1st time to find novel interacting partners. Need protocol for that along with all necessary precautions to be taken.

31 December 2012 4,159 2 View

Can anyone tell me if it's possible to modify mbSUS (mating based split ubiquitin system) to generate and screen cDNA library?

mbSUS has been used successfully by several researchers to screen for protein-protein interaction for membrane proteins but I could not find if anyone has used it to screen cDNA library. if it is...

10 November 2012 832 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View