Does anyone have a protocol/suggestion on leaching out all forms of N from soil or a method to determine content of nitrate, nitrite or any other form of N present in soil?
Suppose I have a wild type and mutants A, B and AB. I take control (untreated) as well as treat each of them (i.e the WT and the mutants A,B and AB) with some treatment/s and perform RT-qPCR for...
10 November 2014 5,008 9 View
Suppose I have around 20 differentially expressed genes for which RT-qPCR data has been generated, and the samples are a wild-type plant and a knock-out mutant. Which statistical analysis should...
09 October 2014 2,839 17 View
We need to validate our microarray data for a few down-regulated genes which show log2FC values near -7. Can qPCR detect this level of down-regulation?
03 April 2014 3,403 11 View
See above
03 April 2014 5,598 2 View
I am attempting GST-pull down assay for finding unknown interacting partners using GST-fusion protein. Normally bait control, prey control, resin control are taken but I am planning to take also...
31 December 2013 9,894 3 View
I have previously used the qiagen plant RNeasy minikit and that works very well, but I would like to know about the performance of this one.
11 December 2013 1,494 2 View
Can anyone suggest a reliable method to estimate nitrate content in plant tissue other than kjeldahl method?
04 May 2013 5,930 8 View
I am trying to express a plant membrane protein using pGEX vector in BL21 strain. I could express the protein but all went into the pellet fraction. Should I purify the protein from the pellet...
01 February 2013 3,058 14 View
I am planning to do co-IP for 1st time to find novel interacting partners. Need protocol for that along with all necessary precautions to be taken.
31 December 2012 4,021 2 View
mbSUS has been used successfully by several researchers to screen for protein-protein interaction for membrane proteins but I could not find if anyone has used it to screen cDNA library. if it is...
10 November 2012 724 1 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...
02 March 2021 752 2 View