What kind of statistical analysis should be performed?

Suppose I have a wild type and mutants A, B and AB. I take control (untreated) as well as treat each of them (i.e the WT and the mutants A,B and AB) with some treatment/s and perform RT-qPCR for...

10 November 2014 5,008 9 View

What kind of statistical analysis should be carried out for RT-qPCR data, which has been used to validate microarray data?

Suppose I have around 20 differentially expressed genes for which RT-qPCR data has been generated, and the samples are a wild-type plant and a knock-out mutant. Which statistical analysis should...

09 October 2014 2,839 17 View

What is the maximum log2fold change detectable for repressed genes using qPCR?

We need to validate our microarray data for a few down-regulated genes which show log2FC values near -7. Can qPCR detect this level of down-regulation?

03 April 2014 3,403 11 View

Can anyone suggest a tool which can take AGI/TAIR ids as input and map them onto actual pathway maps?

See above

03 April 2014 5,598 2 View

Can someone suggest the controls for GST pull down?

I am attempting GST-pull down assay for finding unknown interacting partners using GST-fusion protein. Normally bait control, prey control, resin control are taken but I am planning to take also...

31 December 2013 9,894 3 View

Has anyone used the Xcelris plant RNA isolation minikit?

I have previously used the qiagen plant RNeasy minikit and that works very well, but I would like to know about the performance of this one.

11 December 2013 1,494 2 View

Method/protocol needed

Does anyone have a protocol/suggestion on leaching out all forms of N from soil or a method to determine content of nitrate, nitrite or any other form of N present in soil?

02 March 2013 4,205 0 View

Expression of membrane protein

I am trying to express a plant membrane protein using pGEX vector in BL21 strain. I could express the protein but all went into the pellet fraction. Should I purify the protein from the pellet...

01 February 2013 3,058 14 View

Where can I find a protocol for Co-IP for protein-protein interaction?

I am planning to do co-IP for 1st time to find novel interacting partners. Need protocol for that along with all necessary precautions to be taken.

31 December 2012 4,021 2 View

Can anyone tell me if it's possible to modify mbSUS (mating based split ubiquitin system) to generate and screen cDNA library?

mbSUS has been used successfully by several researchers to screen for protein-protein interaction for membrane proteins but I could not find if anyone has used it to screen cDNA library. if it is...

10 November 2012 724 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View