Has anyone used the Xcelris plant RNA isolation minikit?

More Navjyoti Chakraborty's questions See All

What kind of statistical analysis should be performed?

Suppose I have a wild type and mutants A, B and AB. I take control (untreated) as well as treat each of them (i.e the WT and the mutants A,B and AB) with some treatment/s and perform RT-qPCR for...

10 November 2014 5,117 9 View

What kind of statistical analysis should be carried out for RT-qPCR data, which has been used to validate microarray data?

Suppose I have around 20 differentially expressed genes for which RT-qPCR data has been generated, and the samples are a wild-type plant and a knock-out mutant. Which statistical analysis should...

09 October 2014 2,954 17 View

What is the maximum log2fold change detectable for repressed genes using qPCR?

We need to validate our microarray data for a few down-regulated genes which show log2FC values near -7. Can qPCR detect this level of down-regulation?

03 April 2014 3,543 11 View

Can anyone suggest a tool which can take AGI/TAIR ids as input and map them onto actual pathway maps?

See above

03 April 2014 5,708 2 View

Can someone suggest the controls for GST pull down?

I am attempting GST-pull down assay for finding unknown interacting partners using GST-fusion protein. Normally bait control, prey control, resin control are taken but I am planning to take also...

31 December 2013 10,006 3 View

Nitrate estimation in plants?

Can anyone suggest a reliable method to estimate nitrate content in plant tissue other than kjeldahl method?

04 May 2013 6,048 8 View

Method/protocol needed

Does anyone have a protocol/suggestion on leaching out all forms of N from soil or a method to determine content of nitrate, nitrite or any other form of N present in soil?

02 March 2013 4,335 0 View

Expression of membrane protein

I am trying to express a plant membrane protein using pGEX vector in BL21 strain. I could express the protein but all went into the pellet fraction. Should I purify the protein from the pellet...

01 February 2013 3,164 14 View

Where can I find a protocol for Co-IP for protein-protein interaction?

I am planning to do co-IP for 1st time to find novel interacting partners. Need protocol for that along with all necessary precautions to be taken.

31 December 2012 4,159 2 View

Can anyone tell me if it's possible to modify mbSUS (mating based split ubiquitin system) to generate and screen cDNA library?

mbSUS has been used successfully by several researchers to screen for protein-protein interaction for membrane proteins but I could not find if anyone has used it to screen cDNA library. if it is...

10 November 2012 832 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Prakash Chandra

we use RNA experss reagent of Himedia ... and we are getting good results from plants like Brassica And Finger millet...

Gopal Kumar Prajapati

Dear Novjyoti,

In Comparison to Quizen, Xcelris have low fidelity , but it also gives the good result,,

follow the proper protocol of the kit... sharp handling will give good result...