Dataset size and the need for cross-validation (medical domain)?

I am currently envisioning a deep-learning model that classifies pulse waveforms (normal vs. pathologic). At this time, the no. of pulse dataset is about 2,000,000, and the balance of normal and...

31 July 2020 6,713 3 View

What will be coefficient of friction for mild steel tube when axially impacted by a mild steel projectile ?

i am doing a study related to axial compression on mild steel .i need coefficient of friction value when mild steel tube axially compressed by a large mild steel projectile.i am using abaqus FE...

13 April 2020 259 4 View

What is the fracture energy of mild steel ? how can get from true stress and true strain graph?

I am using abaqus /explict for modeling with j c model for target material is of mild steel and i required fracture energy in in damage model

05 March 2020 4,241 2 View

What is the mass of CO2 at 1 atm pressure and RT ?

I want to calculate the Sheldon E factor for (environmental and sustainability) of reaction. In my reaction i have used 1 atm CO2 as one of the reagent so we need the amount of CO2 in (gm/ mg)...

30 July 2018 7,927 2 View

How can i use EMBL pride inspector?

Hellow everyone. I am supposed to use EMBL pride proteomics documents. But I don't know how to use EMBL pride inspector program. even there is no file to introduce inspector program. Up to now, I...

29 May 2018 4,754 2 View

Has anybody used the user subroutine for single crystal plasticity provided by Prof. Kysar (originally by Dr. Huang) for studying texture evolution?

I am using the subroutine for polycrystal plasticity but I am not able to obtain right deformation texture. if anybody has already used it for the said purpose, would be delighted to seek guidance...

13 March 2017 8,400 3 View

How to stain macrophage, especially mouse macrophage j774a, in relation to immunohistochemistry?

16 October 2016 8,317 2 View

Isn't there any other way to collect a flagella of salmonella typhimurium?

09 June 2016 1,452 3 View

I am interested in Signal Processing, Control Systems and Power Systems. Any particular area you want to suggest to combine all of three above ?

06 October 2014 3,351 9 View

How might we be able to preserve the past through old or traditional buildings?

How can people keep the past when the city is being moved forward?

25 November 2013 1,787 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View