Dear researchers,
Based on the link below:
https://www.youtube.com/watch?v=QThSKVXG7qM&t=1s
I have some questions:
1) for extraction solvent, May I know what types of water and methanol should I use? Distilled water? MilliQ Water? MS grade Methanol (with suitable IS mix 100 ng)? Common methanol?
2) Cells required: 2 million (10e6) cells for metabolite extraction.
How much (volume of cultured cells) should I take?
Thank you.