Dear researchers,

Based on the link below:

https://www.youtube.com/watch?v=QThSKVXG7qM&t=1s

I have some questions:

1) for extraction solvent, May I know what types of water and methanol should I use? Distilled water? MilliQ Water? MS grade Methanol (with suitable IS mix 100 ng)? Common methanol?

2) Cells required: 2 million (10e6) cells for metabolite extraction.

How much (volume of cultured cells) should I take?

Thank you.

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