I'd like to measure levels of NF-κB activation. Is nuclear localization of NF-κB sufficient evidence of activation in human cells?
Austin Morrissey A gene reporter experiment, which introduces an exogenous NF-B consensus promoter sequence connected to a measurable reporter gene' such as luciferase, is a typical approach for measuring NF-B activity at the transcriptional activation level.
A gene reporter experiment, which introduces an exogenous NF-B consensus promoter sequence connected to a measurable reporter gene' such as luciferase, is a typical approach for measuring NF-B activity at the transcriptional activation level.
NF-B targets genes involved in the onset and progression of inflammation. NF-B is a transcription factor that may be activated. After activation, it can control inflammation by activating the transcription of several genes.
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