Measuring cf-mtDNA on human plasma?

15 July 2020 1 9K Report

Hello.

I read that it's possible to compare the ct values of the nuclear gene to mtDNA gene. Does someone have experience with it? How do I need to plane my qPCR reaction?

How I'm doing the analysis in such a case?

I have purified plasma from human blood. After clearing all the cells and cell debris.

Thank you!

Pradyumna Kumar Mishra

Dear Linoy Israel,

Requesting you to please have a look at the following publications (from our Lab):

Article Impairment of Mitochondrial-Nuclear Cross Talk in Lymphocyte...

Article Ultrafine particulate matter impairs mitochondrial redox hom...

Article Occult hepatitis C virus elicits mitochondrial oxidative str...

Hope this helps.

Best - Pradyumna

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