I am planning to identify specific innate immunity biomarkers in patients with BKC form tear samples. Can anyone advise which biomarkes are most commonly expressed (e.g. alpha-defensines, beta-defensine-2, cathelicidin (LL-37)) and what methods should I use to measure them (tear sampling)?
If there is sufficient sample volume and there is an access to Multiplex assay, analyze all (including TNF-alpha, IL-6, IL-1beta etc).
This depends on the type of immune cells you expect. I would look at IL-8 and MPO for neutrophils. ECP, EDN, IL-5 for eosinophils, Granzyme B for e.g. NK cells, histamine for basophils and mast cells. To sample tears you can use schirmer strips and then you can extract the analytes.
@DanielStrasser @DivakerChoubey Thank you for the useful information
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