Measurement methods of Yeast vitality

More Marco Eigenfeld's questions See All

¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View

How to create parameter and coordinate files for ligand in amber when FATAL error (maybe due to duplicate bond specifications) happens?

Hello, I'm trying to create parameter and coordinate files for a drug (PRG-A01) found on the following page: http://www.probechem.com/products_PRG-A01.html I used the guide made by Michael...

17 July 2024 3,472 3 View

I need help to write a good simulation of the solar system and its travel around the galaxy.Can you help me finish the code?

This is the code I have. I would need: An initial configuration (position and momentum), the orientation of the ecliptic with respect to the direction of travel by the sun, The orientation of the...

09 July 2024 6,063 2 View

?como elimino articulos que no son propios?

eliminoar articulos que son de tu autoria

05 July 2024 2,107 1 View

Thin DNA gel loading sample tips?

I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...

01 July 2024 2,124 2 View

How to export publications to LinkedIn?

Does anyone here know how to export publications (from ResearchGate, ORCID, Google Scholar or EndNote) to a LinkedIn profile? I would like to avoid adding each paper manually. Thanks.

12 May 2024 9,307 4 View

Mini-prepped Plasmid Uncut DNA runs way higher than cut DNA ?

Hello all, I have a mini-prepped DNA that I am trying to digest with EagI. After doing the digestion it looks like the reaction with the restriction enzyme maybe linearizing my plasmid as it...

08 May 2024 1,073 3 View

Is possible to dock metals (metal ions) with an apo-protein using MOE?

Hello I need to perform a docking between some metal ions and a protein, in order to study the stability of that protein before and after the metalation. Which software can be used to achieve that...

08 May 2024 3,269 4 View

Am I wrong when I correct Einstein's Relativity by addressing Einstein's error in the Twin Paradox?

https://www.linkedin.com/posts/quantuniverse_revisiting-the-twin-paradox-or-where-physics-activity-7194380109494079489-qVYV?utm_source=share&utm_medium=member_desktop This is the first of a...

08 May 2024 407 49 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

H-index issues?

Hi Sir/Madam, I would like to know why my h-index is stuck to 15 despite the number of new publications added. Could you explain me how marks are granted. Best, Daniel

28 July 2024 2,607 2 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View

Refractive index in the W-band (75-110 GHz) of some material (Teflon - PTFE) ?

08 July 2024 9,140 1 View

Why my yeast colony PCR gel has one specific band , but the size is incorrect even when the plasmid DNA that is originally transformed is correct?

The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...

01 July 2024 2,356 6 View

What are these small structures found when PBMC cells are isolated?

Image attached is at 20X. Cultured media for checking presence of bacteria and yeast and both tests were negative.

27 June 2024 7,473 4 View

Why did plantations increase in the South and why plantation industry is vital for South India?

26 June 2024 6,547 0 View