Measure ROS levels in zebrafish larvae

More Prabha Ranasinghe's questions See All

Respected academician, I did staining of dormant and non-dormant seeds. Dont know how to explain these pics? please help ?

Peroxidase activity 📷📷 Dormant seed Non dormant seed

17 April 2024 7,384 2 View

Cultural validation of a tool for immigrants?

Hi, I have an inventory designed for USA . I want to use it for immigrants in UK. Should I have to validate it for each of subgroups like for Indians, Chinese , Thai and etc of my selected population?

28 January 2024 6,744 2 View

How do you calculate the fractional inhibitory concentration index of two combinations of antibiotic and plant extract, when their MICs are unknown?

For example, antibiotic X's MIC is reported as >32 µg/ml and MIC plant extraction is reported as >10 µg/ml. what value should we used to calculate the FIC according to the equation?...

07 August 2023 9,626 3 View

What would be the best post hoc analysis following the significant three-way interactions in ANOVA using linear mixed effect model?

In my study, I have ten dependent variables (DVs) and three independent variables (A.B, and C) *A (2 levels) *B (3 levels) *C (2 levels) My research question is how the three-way interactions of...

27 July 2023 3,455 4 View

What is the difference between hypothesis and predictions in a research study? Are there any good resources to follow for writing good hypothesis?

I am currently conducting a research study on the microbial N-cycle, and I would like to create some reasonable hypotheses for my research. However, I sometimes find it challenging to distinguish...

15 June 2023 3,667 1 View

How can chromosomes be isolated for observation using a microscope?

I treated cultured cells with Colchicine (0.5ug/mL) overnight, isolated the cells, and treated them with hypotonic solution (0.075M KCl). After stopping the reaction with Carnoy's fixative...

06 April 2023 10,069 0 View

What is the optimal sample size for criterion validity (To compare a 36 item PA scale with a pedometer)?

I am adapting and validation YPAS to Sri Lanka. Need to know the optimal sample size for criterion validity of YPAS by comparing it to Omron HJ0325 pedometer. TIA

03 April 2023 3,712 3 View

Using years in mutiple regression?

I have dengue incidences as outcome and as independat nvariables time, rainfall, wind flow. Time is as years. Can I do multiple regression for that

17 December 2022 5,606 4 View

Can I use sen slope to analyse trends in median prices?

I have a data base on price changes over years.Can i use sen slope to analyse trends in median prices?

22 September 2022 630 2 View

Are there any validated tools to assess 7 features of effective communication?

I am looking for a tool to assess effective communication 7 pillars in a communication of physicians. Is there any validated tools?

30 November 2021 1,236 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

Can anyone recommend a brand of cas9 protein that can be used for in-vivo editing in model animals?

zebrafish and mouse

19 July 2024 9,158 1 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

Mrinalini Sharma

You can follow the given link for details: https://doi.org/10.1016/j.chemosphere.2018.01.045

Muthulakshmi Sellamani

Hi Prabha,

Please check the link below. This protocol will help you out.

Article Zearalenone induced embryo and neurotoxicity in zebrafish mo...

Sadiq S Mareai

i think this paper will help you out..

Kheireddine Ouali

this article could help you