Prabha Ranasinghe

4 Questions 3 Answers 0 Followers

Questions related from Prabha Ranasinghe

Toluidine blue protocol ?

Can anyone please provide me the toluidine blue protocol for zebrafish neuronal cell staining?

30 January 2018 2,184 2 View

Can any one explain me the underlying mechanism (theory behind) in each step of acylatedtubulin method?

I would like to know what role each reagents (such as Prot k; PBST; PBS; Primary antibody; secondary antibody) play in the acylated tubulin method. And also can any one share the correct protocol...

30 October 2017 7,711 2 View

Two separate bands for PCR products of a same gene on a gel!

Hi Why two different bands appear for PCR products of a same gene, when run them in a same gel, same time (master mix were prepared using same reagents and Cdna). I had quadruplicates in PCR run...

01 January 1970 4,504 3 View

Measure ROS levels in zebrafish larvae

Hello I am interested in assessing/quantifying xenobiotic induced ROS levels in zebrafish larvae. Can anyone please let me know for a reliable protocol. Thanks

01 January 1970 1,065 4 View