Our lab is trying to measure the levels of Fascin1 protein in serum samples of cancer patients. There is a commercial kit to measure the levels of Fascin1 protein in serum samples by ELISA. This kit is from Cloud-Clone Corp. and it has worked... poorly. Most of the times, I can't even get the pattern curve. We use other ELISA kits to other purposes and they work fine, so it's not a user or equipment problem. I have been looking for other commercial kits but I have found none different from that one.

There is little literature about this, so if anyone is working with this or having the same problem, I'd like to know, because I feel kinda frustrated.

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