Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

RDF calculation of water inside the Carbon nanotubes?

I am trying to calculate the rdf of water molecules present inside the Carbon nanotube. I tried to do the indexing first using gromacs tool gmx selection. But when it comes to calculating rdf, I...

17 February 2021 1,066 3 View

Can anyone suggest some leading universities worldwide researching on nanotechnology for postdoc?

My area of research is synthesis of metal nanoparticles and their applications focusing on protein extraction from complex biological mixtures using SDS page gel electrophoresis technique. Please...

09 February 2021 2,941 3 View

How we reduce the Dyes in Botanicals like cotton pods(dry)?

For Example: If we take the botanical (Amra Pods) and we are using the dyes to give color to Amra pods . Here I take Acrylic Red(Dye). Im using 100% dye to amra pods to get it colored. Is there...

31 January 2021 1,079 1 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

How does PEG lower the evaporation rate of water?

04 November 2020 4,643 6 View

What are your experience with 96-Transwell formats?

I am planning to use a 96-well format for an experiment as this will greatly improve throughput and expedite treatments. I will be using Caco-2 cells cultured apically. I need them to form...

02 November 2020 5,287 3 View

How monolayer graphene can be grown on copper foil via LPCVD technique?

Please tell me about the rate (in SCCM unit) of the flow of gases and pressure inside the tube.

12 October 2020 8,315 3 View

A good pre-embedding immunogold electron microscopy protocol?

07 September 2020 5,331 2 View

How to find a surface area for a material with isotherm type 3?

Hello everyone I would like to ask a way to evaluate a precise, reliable and accurate surface area of a material. I used a nitrogen adsorption-desorption analysis on my material, a nanocarbon,...

04 August 2020 7,708 8 View

Could anyone please tell me how to make monolayer graphene oxide from graphene oxide solution?

I have a bottle of monolayer graphene oxide (GO) water solution. The concentration is about 2 mg/ml. But I don't know how to get a uniform monolayer or several layers of GO on a piece of 4 cm*4cm...

12 March 2020 2,800 9 View

Caco-2 differentiation on Transwell vs plastic microplate, what are differences ?

Hello, I'm culturing Caco-2 cells in 96 wells plastic microplate and 24 wells Transwell inserts to work on differentiated monolayers. I've heard some information as Caco-2 differentiation is...

10 February 2020 1,898 2 View

Protocol for Giemsa or H&E stain on cell culture?

Does anyone have a protocol for performing morphology staining of monolayer cells in culture? For cell cultures- are Giemsa staining or H&E staining the best options? I only want to visualize...

09 February 2020 9,225 3 View

How can I avoid the wrinkling of monolayer hBN onto Silicon substrate while annealing?

I'm trying to transfer a monolayer of CVD grown hBN from Cu foil to a target substrate ( in this case, a Si substrate with micro wells. )To improve the adhesion between hBN and So substrate, I'm...

06 February 2020 7,941 1 View

What is the best method for growing neural stem cells on glass coverslips/slides for immunocytochemistry?

I've been working with murine neural stem cells as a 2d monolayer which is achieved by adding laminin (ECM) directly to the culture media, without the need to coat the TC plastics. However, I've...

04 February 2020 8,172 2 View