I am planning to seed HUVECs on matrigel coated wells to do an in vitro tube formation assay. However, I am not sure how to proceed. Any help provided will be much appreciated.
Thank you.
Hello Lian Bonnici
I have attached two links below. You may follow any one of these for the invitro tube formation assay using HUVECs.
Article In vitro angiogenesis: Endothelial cell tube formation on ge...
https://www.corning.com/catalog/cls/documents/protocols/protocol_DL_030_Endothelial_Cell_Tube_Formation_Assay.pdf
Best.
thank you very much. I really appreciate your help Malcolm Nobre .
Hi in case you plan to quantify the HUVEC tube formation assay, here is the link for quantification using ImageJ. Hope it helps you.
https://www.youtube.com/watch?v=sQLYoJxgUCk
Thank you very much.Nandaraj Taye
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