Been facing some problems with cell culture, and hoping to search for suggestion
I'm using a cell line, C2bbe1, which is a subclone of caco-2 cells
And have been trying to induce its inflammation with LPS
I've tried the LPS dosage from 1~100 microgram/L for 24h and 48h
And as expected that non of the dosage will cause cell death nor damage
So I've changed to NO assay by using Griess reagent,
to examine its nitric oxide production in the medium
but non of the dosage were found to have any NO production in the medium
Hoping to search any advisory in how to determine whether my cells are in inflamed state
Cell: C2bbe1
Seeding density: 3*10^5 per well in 6 well plate
Time: after cells were seeded for 48~72 hours, confluency reach about 50~60%, LPS was introduced for 24~48H
Article LPS-stimulated iNOS Induction is Increased by Glucosamine Un...
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