Dear Friends,
5 days back I have done my transformation experiment in yeast and I spread the suspension in 1/10, 1/100 and 1/10000 dilution. Only in 1/10 dilution plate I have got two colonies.
In the last step of transformation mentioned in Y1H clone tech kit, cell should get suspended in 0.9% NaCl solution. I use to store my rest of culture in 4 degree after use.
I am thinking to reuse the same culture on fresh plate in 1/5 and 1/2 dilution. Because there might be possibility of low cell numbers in my previously diluted ratio.
Can some one suggest here that transformed cells stored in 4 degree can be again used after 5 to 6 days?