Amber Gupta

9 Questions 3 Answers 0 Followers

Questions related from Amber Gupta

May I proceed with my transformed 4 degree stored yeast cells again on culture plate?

Dear Friends, 5 days back I have done my transformation experiment in yeast and I spread the suspension in 1/10, 1/100 and 1/10000 dilution. Only in 1/10 dilution plate I...

12 July 2020 1,841 0 View

Trouble shooting with plasmid visualization on agarose gel from E.coli ??

Dear Friends, I am trouble shooting since a long for isolation of my cloned construct in the strain E.oli DH-5 alpha. I have tried Qiagen kit but yield is very low to...

24 November 2019 9,648 2 View

Solution to overcome the diffuse band from SDS-PAGE protein gel and can it be used for western blot?

I have run my Crude extract protein from rice shoot sample and as per my observation I feel that my protein is getting diffused as lower part of my running gel. The gel solutions are freshly...

07 July 2018 2,043 3 View

The staining method to show the differentiation of acidity (low pH) from the cytosole to Vacuole or any other intracellular organelle?

Dear Colleagues, I am planning for an experiment to show the acidity in cytosole in respect to vacuolar (to detect the H+ ion concentration outisde of vacoule) system...

07 October 2017 1,444 0 View

HKT mechanism in rice plant?

Dear Friends, I have confusion regarding HKT transporter activity for Na+/K+ ions in rice plant under salinity. As in most of the literature it is mentioned that mainly...

24 July 2017 9,952 0 View

How to engineer cDNA with His TAG sequence when the cloned cDNA seq. is not known and have to analyze under prey vector in yeast one hybrid assay?

Dear Friends, I have one query regarding ligation of his tag sequence with unknown cDNA sequence that has to be cloned under prey vector for yeast one hybrid assay. How to...

07 March 2017 620 0 View

Image of rice sample isolated RNA, getting lower bands of RNA is more intense?

Hello everyone, Since few time we are isolating RNA by Sigma Trizol method from root and shoot of our rice sample and still we are confusing that why we are getting much...

27 August 2016 1,779 1 View

Has anyone tried with more than 100 bp cloning in PAbai vector supplied with yeast one hybrid clonetech kit

Dear Friends, I am planning to do yeast one hybrid experiment to analyze one of our Oryza Sativa (rice) promoter sequence through Yeast one Hybrid kit supplied by...

01 January 1970 7,506 2 View

Purified cDNA amount trouble shoot for Yeast one hybrid- clone tech library analysis.

I would like to share my today's experiment gel image for cDNA purification. The Lane 3 corresponds to purified cDNA amount (purified through chromo spin column TE-400) while lane 5 corresponds...

01 January 1970 9,507 0 View