7 Questions 3 Answers 0 Followers
Questions related from Amber Gupta
Dear Friends, 5 days back I have done my transformation experiment in yeast and I spread the suspension in 1/10, 1/100 and 1/10000 dilution. Only in 1/10 dilution plate I...
12 July 2020 1,796 0 View
Dear Friends, I am trouble shooting since a long for isolation of my cloned construct in the strain E.oli DH-5 alpha. I have tried Qiagen kit but yield is very low to...
24 November 2019 9,572 2 View
I have run my Crude extract protein from rice shoot sample and as per my observation I feel that my protein is getting diffused as lower part of my running gel. The gel solutions are freshly...
07 July 2018 1,968 3 View
Dear Colleagues, I am planning for an experiment to show the acidity in cytosole in respect to vacuolar (to detect the H+ ion concentration outisde of vacoule) system...
07 October 2017 1,423 0 View
Hello everyone, Since few time we are isolating RNA by Sigma Trizol method from root and shoot of our rice sample and still we are confusing that why we are getting much...
27 August 2016 1,749 1 View
Dear Friends, I am planning to do yeast one hybrid experiment to analyze one of our Oryza Sativa (rice) promoter sequence through Yeast one Hybrid kit supplied by...
01 January 1970 7,458 2 View
I would like to share my today's experiment gel image for cDNA purification. The Lane 3 corresponds to purified cDNA amount (purified through chromo spin column TE-400) while lane 5 corresponds...
01 January 1970 9,478 0 View