We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are uprooted and which microtomy technique do you suggest in order to study the anatomical details after the sectioning of roots.
In theory, I should be able to answer this question, since my Ph.D. was on the anatomy of roots from different species of plants, but that was back in prehistoric days. What anatomical details are you looking at? I was looking at suberin deposition. I could clear and stain roots (with sudan stains, trypan blue and toluidine blue) and for most of it, didn't need thin sections at all. When I did, for use under a fluorescent microscope, I made hand sections, because I was really good at hand sections.
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