I interested in gene expression and cervical cancer and I needed fresh biopsy tissues (no paraffin embedded block) from lesions of patients in different stages of CIN I, CIN II, CIN III and cervical cancer. The problem is when a biopsy sample taken from patient, this sample was embedding in paraffin for histologically detection which isn’t suit for gene expression. Also, taken out two pieces of sample will so painful and is immoral. I grateful if anyone has experience in it and guided me.
Can you do the testing on a liquid sample such a sample collected with a Pap test sampling device, which is then put into a molecular collection media? Or on cells scraped from an air-dried slide?
Sonya Naryshkin
It may be lower level of detection on Pap test compare to punch biopsy. Also, our plan is to have homogeneous population sampling in order to avoid bias when comparing different group of CINs with normal and cervical cancer group.
Have you considered using Liquid based cytology samples?
This could be a suitable sample since you can make multiple slides from one sample.
I still think a liquid based sample, either a scrape as in Pap test sampling, or maybe some kind of irrigation of the cervix just prior to the biopsy, might work for you. It's worth a try.
We think that it may be more valuable to study gene expression in tissue samples and therefore we need a colonoscopy-derived biopsy.
We are coordinating with specialists to take the biopsy from patients with consent, if it doesn't work then we focus on the liquid-based sample as Sonya Naryshkin said.
Thank all.
If that is the approach you are considering it will be wise to take both LBC sample and biopsy from each patient and conduct a comparative study. You may find that the two samples yield the same results. Hit two birds with one stone.
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