Data signal through a twisted pair cable by using different color codes will work ?

will a Data signal through a twisted pair cable by using different color codes will work ?. I am using a shielded twisted pair cable for my Mod bus device. Instead of same color pairs for Data...

02 May 2019 6,361 3 View

What is the reason of decrease the bandgap when decrease the cystallite size?

I prepared nanorod structured undoped (pure) oxide thin film by spray pyrolysis method function of different substrate temperatures (STs). I found that decrease the bandgap when decreases the...

08 August 2017 5,868 21 View

How to lyse bacteria to extract dsRNA using Trizol?

05 June 2017 238 4 View

Is there any way to get individual dTTP from oligo dT?

22 March 2017 1,051 4 View

Can anyone suggest a good method/paper to mass produce TEV protease ?

I am planning to produce TEV protease in my lab because I will need a lot of the protein in order to cleave the fusion tag off the recombinant proteins which I will be producing in E. coli. Do...

03 November 2016 6,281 3 View

How in-cylinder gas temperature is calculated from the in-cylinder gas pressure in a diesel engine?

Suggest suitable formulae or method to calculate the in-cylinder gas temperature in a diesel engine from the measured in-cylinder gas pressure during combustion.

01 November 2016 9,653 8 View

Is the numerical value of positron diffusion constant different from surface to bulk of the solids?

If I consider positron diffusion length L+ is constant through the solid. But by VEPFIT I am getting two layer (surface and bulk) of the solid

02 February 2016 1,270 1 View

Is there a colorimetric assay to quantify terpenoids from plant extract? Which is the best way to quantify terpenoids apart from GC-MS analysis?

Apart from GC-MS analysis which method would be best to quantify terpenoids from plant extract?

27 March 2015 7,764 8 View

How can one obtain a better yield from Palm oil fractionation? Is 85% achievable on a PO IV of 53?

What would the cycle time be?

02 December 2014 8,369 3 View

Need help with writing a fortran code linked with abaqus,

For every load step, fortran code is complied from beginning, when we link with abaqus. My question is: if, for example, I am solving for a(t) and 't' is time at current load step and I want a(t)...

14 August 2013 6,582 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View