17 Questions 12 Answers 0 Followers
Questions related from Vrushali Kashinath Chimankar
Hello everyone, I'm planning to conduct an experiment to identify the presence or absence of Y chromosomes in various subsets of immune cells from clinical PBMC samples. However, as a novice in...
15 February 2024 5,805 1 View
Hi all, I am interested in knowing if there are antibodies that I can use to detect fibrosis in blood cells using flow cytometry. Can anyone with expertise in immunology and flow cytometry please...
30 November 2023 449 1 View
I have some WGS data from mouse models and I am trying to use annovar for gene annotation. However I am getting stuck at the step where I download the mouse database for my analysis. I get this...
24 June 2020 3,558 0 View
My lab is looking into buying following histology equipment and I was wondering which company offers the best equipment, Thermofisher or Leica? Fully automated microtome Tissue...
13 February 2019 7,516 9 View
Hi all, Is it possible to extract RNA from formalin fixed paraffin embedded tissue sections which would be good enough in quality and quantity for RNA sequencing? I have some slides with mouse...
21 January 2018 8,320 1 View
Hello, Can someone suggest me the recipe for making Eosin solution from eosin y (sigma;dye content 99% ). I followed the recipe - 1gm Eosin in 20ml water and 80ml 95% ethanol but the eosin powder...
03 February 2017 4,295 3 View
I intend to validate the results of gene expression data from RNA sequencing/ microarray using Immunohistochemistry. In short I want to check the expression pattern on a protein level. How many...
10 January 2017 5,982 3 View
I want to align promoter sequence of a small T antigen and large T antigen of virus (merkel cell polyomavirus). I am aware that for human genome we generally subtract and add 1 kb from genome to...
02 October 2015 9,513 1 View
I am reading papers on salivary biomarkers and was wondering if the supernatant obtained after centrifugation of saliva contain soluble proteins? Thank you
13 July 2015 4,805 2 View
DAB is a carcinogen and so we use gloves while handling it. Do you discard the gloves or can you reuse. As per my understanding we should discard it but I have seen that in some labs they just...
08 May 2015 3,547 2 View
I am reading about platinum resistance in ovarian cancer. The papers state that increased Nucleotide excision repair may cause platinum resistance but I am not able to understand how. The platinum...
17 April 2015 7,645 3 View
I came across word phospho mutants in a webpage and I am not quite sure what it means. Can anyone please explain? Thanks :)
09 February 2015 5,410 1 View
I have a exome sequencing data of parents and their child. We are trying to identify the candidate genes responsible for some abnormality.I have never used CLC genomics workbench. Do I need a...
24 October 2014 6,655 2 View
When MSA from T-COFFEE was used all mutations were predicted to be neutral,from CLUSTA-W only few mutations were predicted (2/56)but msa from COBALT predicted results for all the mutations.
08 July 2014 4,777 2 View
I tried using I-TASSER, ROSSETA, and RaptorX but all of these deal with proteins having less that 2000 residues. I am trying to analyze an APC protein which has 2843 amino acids.
28 June 2014 2,971 9 View
What program is best for gene annotation for mouse genome. Annovar or SNPeff or both?
01 January 1970 3,424 1 View
Hello, I am working on lung cancer project and we are planning to perform IHC on tissue sections from mice lung cancer tissue. We are coating the slides with silane. Is it important to clean the...
01 January 1970 5,314 5 View
